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TNCs from cord blood were expanded for 7 days with StemCell2MAX MIX and their number, phenotype and viability was evaluated.
In conclusion, the number of TNCs increased by a factor of approx. 10, but more importantly, the number of CD34+ Haematopoietic Stem and Progenitor Cells (HSPC) were selectively increased by a factor of approx. 100.
Notably, these expanded progenitor cells kept their multipotent differentiation capacity intact, as confirmed by the equal diversity of blood lineages in CFU functional assays.
Benevides, et al.. “Expansion of cord blood cells using a Mix of neurotrophic factors | Quality protocols in Cord Blood Banks", Proceedings of the 9th Int. Meet. Port. Soc. Stem Cells, 2015
Cord blood HSC expansion with RET ligands.
Left: Total nucleated cells from 7 days cultures of HSCs expanded in the presence or absence of RET signals.
Centre: Percentage of transplantation efficiency into irradiated hosts of same cell numbers from cultured cells shown in left panel.
Right: Overall fold increase in expansion of bona fide, ready-for- transplant HSCs
Fonseca-Pereira, et al.. “The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function”, Nature 514, 2014
Hematopoietic stem cells (HSCs) can be obtained from different sources. Common sources include bone marrow, umbilical cords and peripheral blood of a patient or an adult donor.
However, having to obtain HSCs from human tissues makes them scarce and expensive. There is therefore a need for techniques that are able to improve the availability and utility of HSCs in the lab.
StemCell2MAX markets a proprietary mix of neurotrophic factors that specifically control cord blood HSC survival, therefore expanding HSCs while maintaining their stemness.