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Results published by customers

 

TNCs from cord blood were expanded for 7 days with StemCell2MAX MIX and their number, phenotype and viability was evaluated.

 

In conclusion, the number of TNCs increased by a factor of approx. 10, but more importantly, the number of CD34+ Haematopoietic Stem and Progenitor Cells (HSPC) were selectively increased by a factor of approx. 100.

 

Notably, these expanded progenitor cells kept their multipotent differentiation capacity intact, as confirmed by the equal diversity of blood lineages in CFU functional assays.

 

 

 

Benevides, et al.. “Expansion of cord blood cells using a Mix of neurotrophic factors | Quality protocols in Cord Blood Banks", Proceedings of the 9th Int. Meet. Port. Soc. Stem Cells, 2015

20x better results than state-of-the-art

Cord blood HSC expansion with RET ligands.

Left: Total nucleated cells from 7 days cultures of HSCs expanded in the presence or absence of RET signals.

Centre: Percentage of transplantation efficiency into irradiated hosts of same cell numbers from cultured cells shown in left panel.

Right: Overall fold increase in expansion of bona fide, ready-for- transplant HSCs

 

Fonseca-Pereira, et al.. “The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function”, Nature 514, 2014

100x HSC fold increase while keeping multipotent differentiation capacity intact

Background

 

 

Hematopoietic stem cells (HSCs) can be obtained from different sources. Common sources include bone marrow, umbilical cords and peripheral blood of a patient or an adult donor.

However, having to obtain HSCs from human tissues makes them scarce and expensive. There is therefore a need for techniques that are able to improve the availability and utility of HSCs in the lab.

 

StemCell2MAX markets a proprietary mix of neurotrophic factors that specifically control cord blood HSC survival, therefore expanding HSCs while maintaining their stemness.

OPENING UP NEW AVENUES FOR RESEARCH

Relevant publication

  • Benevides, P., Rebelo, R. and Arroz-Madeira, S.. “Expansion of cord blood cells using a Mix of neurotrophic factors | Quality protocols in Cord Blood Banks", Proceedings of the 9th Int. Meet. Port. Soc. Stem Cells, 2015

 

  • Fonseca-Pereira, D., Arroz-Madeira, S., Campos, M., Barbosa, I., Domingues, R. G., Almeida, A. R. M., Ribeiro, H., Enomoto, H., Potocnik, A. and Veiga-Fernandes, H.. “The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function”, Nature 514, 2014

 

  • Almeida, A. R. M., Fonseca-Pereira, D., Arroz-Madeira, S., Ribeiro, H., Labão-Almeida, C. and Veiga-Fernandes, H.. “RET regulates IL-10 production by in vitro polarised T helper 2 cells”, European Journal of Immunology 44, 2014

 

  • Patel, A., Harker, N., Moreira-Santos, L., Ferreira, M., Alden, K., Timmis, J., Foster, K., Garefalaki, A., Pachnis, P., Andrews, P., Enomoto, H., Milbrandt, J., Pachnis, V., Coles, M.C., Kioussis, D., Veiga-Fernandes, H.. "Differential RET signaling pathways drive development of the enteric lymphoid and nervous systems", Science Signaling 5, 2012

 

  • Veiga-Fernandes, H., Coles, M.C., Foster, K.E., Patel, A., Williams, A., Natarajan, D., Barlow, A., Pachnis, V., Kioussis, D.."Tyrosine kinase receptor RET is a key regulator of Peyer's patch organogenesis", Nature 446, 2007

 

  • Danby, R., Rocha, V.."Improving engraftment and immune reconstitution in umbilical cord blood transplantation" Frontiers in Immunology 5, 2014

 

  • Walasek, M.A., van Os, R., de Haan, G.."Hematopoietic stem cell expansion: challenges and opportunities" Annals of the New York Academy of Sciences 1266, 2012

 

  • Mulligan, L.M.. "RET revised: expanding the oncogenic portfolio", Nature Reviews Cancer 14, 2014

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